RESUMO
TRIP13 is a member of the large superfamily of the AAA + ATPase proteins and is associated with a variety of activities. Emerging evidence has shown that TRIP13 may serve as an oncogene. However, the function of TRIP13 in breast cancer (BC) has not yet been elucidated. Here, a variety of bioinformatic tools and laboratory experiments were combined to analyse the expression patterns, prognostic value and functional network of TRIP13 in BC. Multiple databases and immunohistochemistry (IHC) indicated a higher TRIP13 expression in BC tissue compared with normal tissue. TRIP13 was highly expressed in lung metastatic lesions compared with primary tumours in a 4T1 cell implantation BALB/c mouse model of BC. Kaplan-Meier plots also revealed that high TRIP13 expression correlated with poor survival in patients with BC. Furthermore, gene set enrichment analysis revealed that TRIP13 was primarily enriched in the signalling pathway of PI3K-AKT-mTOR. Suppressing TRIP13 could inhibit the expression of related genes, as well as the proliferation and migration of BC cell. Finally, 10 hub genes with a high score of connectivity were filtered from the protein-protein interaction (PPI) network, including MAD2L1, CDC20, CDC5L, CDK1, CCNA2, BUB1B, RAD51, SPO11, KIF11 and AURKB. Thus, TRIP13 may be a promising prognostic biomarker and an effective therapeutic target for BC.
Assuntos
Neoplasias da Mama , ATPases Associadas a Diversas Atividades Celulares/genética , ATPases Associadas a Diversas Atividades Celulares/metabolismo , Animais , Biomarcadores Tumorais/genética , Neoplasias da Mama/genética , Proteínas de Ciclo Celular/genética , Proteínas de Ciclo Celular/metabolismo , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Camundongos , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas de Ligação a RNA/metabolismo , Transdução de Sinais/genéticaRESUMO
In this paper, we demonstrate a tunable periodic hourglass-shaped graphene arrays absorber in the infrared (IR) and terahertz (THz) frequency bands. The effects of graphene geometric parameters, chemical potentials, periods, and incident angles on the pure absorption characteristics are studied by using the Finite Difference Time Domain (FDTD) method. In addition, this paper also analyzes the pure absorption characteristics of bilayer graphene arrays. The simulation results show that the maximum absorption reaches 38.2% for the monolayer graphene structure. Furthermore, comparing the bilayer graphene structure with the monolayer structure under the same conditions shows that the bilayer structure has a tunable dual-band selective absorption effect and has a higher maximum absorption of 41.7%. Moreover, it was found that there are dual-band tunable absorption peaks at 26 µm and 36.3 µm with the maximum absorption of 41.7% and 11%. The proposed structure is a convenient method which could be used in the design of graphene-based optoelectronic devices, biosensors, and environmental monitors.
